【摘要】 为了定向筛选α-糖苷酶抑制剂,建立了抑制α-淀粉酶、蔗糖酶和麦芽糖酶活试验为基础的筛选模型.采用此模型筛选从土壤和植物中分离的放线菌,其中菌株E-3519的发酵液对α-淀粉酶和蔗糖酶有强烈的抑制作用.菌株E-3519无气丝,基丝生长好,偶见孢囊,孢囊孢子游动迟缓;能水解淀粉,硝酸盐还原实验阳性,产生硫化氢,能使牛奶冻化;能利用大多数碳源;对枯草杆菌、大肠杆菌、金色葡萄球菌等无拮抗作用;可产生α-糖苷酶抑制剂阿卡波糖.E-3519主要生化特征及形态学特征与ATCC14539相似,命名为Actinoplanesutahensisvar..更多还原

【Abstract】 To screen α-glucosidase inhibitor, three methods using the inhibitor activity of amylase, sucrase, maltase as models were established. During the screening program of α-glucosidase inhibitor, It was found that, the culture broth of strain E-3519 exhibited amylase and sucrase inhibitory activity. Strain E-3519 produced fine branching mycelium without aerial mycelium, sporangia were rare or absent, spores in sporangium moved slowly. Starch hydrolysis, nitrate reduction, milk peptonization and H2S production were positive. Strain E-3519 could utilize most of carbon sources and the fermentation broth appeared no activities against G+, G-. Acarbose could also be produced during the cultivation. Strain E-3519 was extremely similar to ATCC14539 and was named as Actinoplanes utahensis var.更多还原