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大叶紫薇叶中熊果酸的分离纯化及结构
Isolation and Identification of Ursolic Acid from Lagerstroemia specious L.
【摘要】 利用溶剂萃取、薄层层析对大叶紫薇提取物中三萜化合物进行分离,通过 DSC,UV, IR和HPLC/MS对分离产物中具有降血糖的成分 3 进行结构分析,结果表明:以氯仿∶丙酮为展开剂,可在硅胶板中将大叶紫薇中三萜化合物有效分离。对分离出的成分 3,DSC测定其熔点为 283~287 ℃,焓值为43 727 J/g;UV最大吸收波长为 206 nm; MS测定其相对分子质量为 457; IR光谱符合乌苏果烷型结构.经分析,具有降血糖的成分3即为熊果酸.
【Abstract】 Solvent-extraction and thin-layer chromatography were used to extract and separate triterpene from Lagerstroemia specious L. Structures of the composition #3 were identified by DSC、UV、 IR and HPLC/MS.The results showed that, triterpene can be efficiently separated with thin-layer chromatography using: acetone as the developer. The result also indicated that, the melting point, enthalypy,optimum absorbance wave lenghth, and molccular weight were 283~287 ℃,43.727 J/g, 206 nm, and 45%, respectively. IR spectrum fit that of ursane structure. The result showed compound #3 was ursolic acid.
【Key words】 Lagerstroemia specious L.; ursolic acid; isolation; identification;
- 【文献出处】 无锡轻工大学学报(食品与生物技术) ,Journal of Wuxi University of Light Industry , 编辑部邮箱 ,2005年01期
- 【分类号】TQ464
- 【被引频次】18
- 【下载频次】345