【摘要】 采用硫酸、乙醇等提取,Sephadex G 50 及 RP HPLC技术纯化,从苦瓜中分离出苦瓜多肽 P,又称植物胰岛素,该技术优于过去的薄层层析纯化方法.皮下注射该多肽可降低正常小鼠和四氧嘧啶模型小鼠的血糖,口服无效.经胃蛋白酶、胰蛋白酶水解后,该肽降糖活性丧失.更多还原

【Abstract】 P-polypeptide, that was so called “plant insulin”, was extracted from Momordica charantia seeds with vitriol and ethanol and purified with Sephadex G-50 gel filtration and RP-HPLC. The technique was better than thin-layer chromatography technology. The peptide subcutaneously injected could lower blood glucose in normal mice and alloxan-induced diabetic mice, but not for the oral case. The peptide hydrolyzed by pepsin or trypsin lost its hypoglycemic function.更多还原