【摘要】 目的 :观察血小板源性生长因子 (PDGF)对于培养脐静脉内皮细胞碱性成纤维细胞生长因子 (bFGF)水平的影响 ,探讨PDGF促进新生血管形成的机制。方法 :通过内皮细胞的培养和免疫组织化学等方法检测PDGF对缺氧和常规培养内皮细胞bFGF水平的影响以及不同浓度PDGF促bFGF表达的作用。结果 :缺氧培养与常规培养均能增加内皮细胞胞浆bFGF水平 ,且呈剂量依赖性。结论 :PDGF可能通过直接促血管形成生长因子bFGF的介导作用间接促进新生血管的形成。更多还原

【Abstract】 Objective:To investigate platelet-derived growth factor(PDGF) modulate the expression of basic Fibrolast Growth Factor(bFGF) in human umbilical endothelial cells and PDGF influence mechanism on angiogensis.Method:The level of bFGF of endothelial cell was detected with Immunhistochemical.Results:The results as followed,level of bFGF have changes w hen cell under normal or hypoxia.Conclusion:These manifest shows PDGF promoted angiogensis by i ncreasing bFGF leves of endothelial cells.更多还原