【摘要】 目的　研究sHsps在正常腭发育及腭裂发生过程中的表达情况。方法　在GD10经口一次分别给予实验组孕小鼠80mg kg的视黄酸(RA)、对照组孕鼠等体积的植物油,并分别于GD15 GD17取两组胎鼠的腭,利用RT- PCR方法半定量检测实验和对照组中sHsps的表达丰度。结果　正常腭除Hspb10在GD17不表达外,其余Hsps基因在GD15 - GD17均有明显表达,Hsp2 0、Hsp2 5、Hsp2 7、Hsp32、Hspb2、Hspb3、Hspb7表达丰度基本恒定,Hsp30、Hspb5随胚龄的增加而表达丰度增高,Hsp10、Hsp2 2、Hspb4、Hspb9在GD16出现表达高峰。Hsp30、Hsp32、Hspb4、Hspb10在GD15 - GD17的表达丰度腭裂组明显高于正常组。Hsp10、Hsp6 0、Hspb5、Hspb9在GD15 - GD17的表达丰度腭裂组明显低于正常组。Hspb9、Hspb10在正常腭发育和腭裂发生过程中的表达模式明显不同。结论　15种Hsps除Hspb10在GD17不表达外,其余在GD15 - GD17正常腭发育过程中均有明显表达,但不同Hsps的表达特点有所不同;Hsp30、Hsp32、Hspb4、Hspb10在腭裂发生中可能主要起应激保护反应,Hsp10、Hsp6 0、Hspb5、Hspb9、Hspb10可能与腭裂的发生有关。更多还原

【Abstract】 Objective To study the expression of sHsps in normal palate and cleft palate during mouse embryogenesis. Methods At GD10, gestational mice of the treatment and the control were administered with 80mg/kg retinoic acid and the same volume vegetable oil separately, and the normal palate and cleft palate of embryos were harvested in GD15-GD17. The relative abundance of sHsps of all samples was measured by reverse transcript polymerase chain reaction (RT-PCR). Results In the normal limbs, except that there is no expression of Hspb10 at GD17, all the other Hsps expressed obviously in GD15-GD17. To the normal palates, the expressional abundance of Hsp20、Hsp25、Hsp27、Hsp32、Hspb2、Hspb3、Hspb7 was stable, that of Hsp30、Hspb5 was increased following the embryos aging, and the expressional peak of Hsp10、Hsp22、Hspb4 occurred at GD16. In GD15-GD17, the expressional abundance of Hsp30、Hsp32、Hspb4、Hspb10 of the cleft palates was higher than that of the normal palates, but the expressional abundance of Hsp60、Hspb5、Hspb9 of the cleft palates was lower than that of the normal palates. The expressional models of Hspb9、Hspb10 of the normal palates were different from those of the cleft palates obviously. Conclusion Except that there is no expression of Hspb10 at GD17, all the other Hsps expressed obviously in GD15-GD17 during normal clefts’ development. To different Hsps, there is different expressional characteristic. Hsp30、Hsp32、Hspb4、Hspb10 maybe play a protective role in the stress action of cleft palate, and Hsp10、Hsp60、Hspb5、Hspb9、Hspb10 were maybe relative to cleft palate.更多还原