【摘要】 结核病仍旧威胁着全球人类健康,中国是结核病高发国家之一,寻求新的药物和疫苗势在必行。随着对噬菌体研究的深入,分枝杆菌噬菌体成为结核病新型药物发现和药敏实验的研究热点之一。噬菌体进入宿主菌体内,以裂解和溶源两种途径进入循环。以分枝杆菌的溶源性噬菌体为例,综述了分枝杆菌噬菌体整合和裂解分子机理。分枝杆菌溶源性噬菌体的整合需噬菌体基因组的附着位点attachment site(attP),宿主菌分枝杆菌基因组的附着位点attachment site(attB),整合酶integrase(Int)和整合宿主因子integration host factor(mIHF)。部分溶源性噬菌体如Ms6进入裂解循环,复制转录组装成新的子代噬菌体,在裂解素(Lysin)和穿孔素(Holin)的协同作用下裂解宿主菌,释放子代噬菌体。目前国内未见对分枝杆菌噬菌体的研究报道。研究分枝杆菌噬菌体整合及裂解机理对结核病治疗新药开发有一定的启示。更多还原

【Abstract】 Tuberculosis remains one of the major threats to public health. China is one of the heavy TB burden countries. Novel drugs and vaccines are urgently needed to combat the increasingly multidrug resistant TB. Mycobacteriophage is one of the hot topic in TB novel drugs discovery and drug susceptibility test. Phages can multiply via two alternative mechanisms: the lytic cycle or the lysogenic cycle. The lytic cycle ends with the lysis and death of the host cell, whereas the host cell remains alive in the lysogenic cycle. Lysogenic mycobacteriophages were intensively studied to elucidate the integration and lysis mechanisms of mycobacteriophage. The integration of mycobacteriophage requires for attP of bacteriopahge genome, attB of Mycobacterium genome, integrase and integration host factor. Some lysogenic phage, eg. mycobacteriophage Ms6,employ lytic cycle, form new phage, lysis host by the cooperation of lysin and holin, and release phages. There is no reports as to the mycobacteriophage unique to China clinical or environmental isolates. Studies on the integration and lysis molecular mechanism of mycobacteriophage might facilitate future new anti-TB drugs development.更多还原