【摘要】 在野生型的圈卷产色链霉菌中 ,增加单个或多个拷贝数的samR ,观察该基因对野生株表型和形态的影响。结果表明 ,samR的拷贝数的增加会使孢子形成提前 ,但是增加单拷贝的菌株与增加多拷贝的菌株所表现的增效作用相同。在大肠杆菌中对samR进行了GST融合表达和纯化 ,通过凝胶阻滞实验证明 ,SamR蛋白可与其上游的调控区特异性结合。由此推测samR是一个自调控基因更多还原

【Abstract】 The previous result showed that samR plays an important role in the development progress of Streptomyces ansochromogenes. It was reported that the differentiation progress of S. ansochromogenes was accelerated by a recombinant plasmid containing anextra copy of samR gene. However, the differentiation progress of S. ansochromogenes was not further accelerated by a multicopy plasmid containing samR gene. Electrophoresis mobility shift assay (EMSA) demonstrated that SamR binds to its own promoter region specifically. All these results hint that samR is an autoregulatory gene in Streptomyces ansochromogenes.更多还原