【摘要】 通过气管内滴注葡聚糖诱导豚鼠气道炎症,研究嗜酸细胞在气道炎症中的意义。用葡聚糖G200(5mg/kg)滴注形成气道炎症,进行支气管肺泡灌洗,通过RTPCR方法,检测气道炎症时肺匀浆Eotaxin基因的表达。结果可见,葡聚糖G200所致豚鼠肺损伤后Eotaxin的mRNA表达水平随葡聚糖G200刺激时间延长而明显升高(P<0.05),同时支气管肺泡灌洗液中白细胞数及上清液中Th2型细胞因子IL4也随之升高(P<0.05);试验中采用甲强龙琥珀酸钠抑制气道炎症,与盐水对照组比较P>0.05。可见,用葡聚糖G200进行气管滴注是形成气管炎症简单而实用的动物模型;Eotaxin在聚集、活化炎性细胞过程中起重要作用;糖皮质激素类药物能有效抑制Eotaxin的表达。更多还原

【Abstract】 Airway of guinea trachea was instilled with Sephadex G-{200} (5 mg/kg) to induce inflammation to study the significance of eosinophils in airway inflammation. Eotaxin gene expression was measured in lung tissue during airways inflammation and then carried out bronchoalveolar lavage (BAL). The results showed that mRNA expression level of eotaxin was elevated obviously (P<0.05) with prolongation of Sephadex G-{200} stimulation after pulmonary damage of the induction. Meanwhile amount of WBC in BAL fluid and IL4 in supernatant were also increased {(P<0.05).} Methylprednisolone sodium succinate was used to inhibit airway inflammation compared with using NS as control group (P<0.05). Therefore, it is simple and practical animal model with tracheal instillation of Sephadex G-{200} to induce airway inflammation, and eotaxin plays an important role in the course of aggregation and activation of inflammatory cells; glucocorticoid drugs can inhibit eotaxin expression effectively.更多还原