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几株光合细菌的分离鉴定及在养殖罗非鱼中的应用

Isolation and Identification of Several Photosynthesis Bacteria and Their Application in the Culture of Tilapia sp.

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【作者】 黄志勇王海胜蒋培霞宋安东刘志培

【Author】 HUANG Zhi-Yong~(1,3 ) WANG Hai-Sheng~(2 ) JIANG Pei-Sia~(2) SONG An-Dong~(2) LIU Zhi-Pei~(1**)(Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080)~(1)(College of Biology and Food Science, Henan Agricultural University, Zhengzhou 450002)~(2) (Savannah River Ecology Lab, University of Georgia, 227 Gateway Dr., SC 29802, USA)~(3)

【机构】 中国科学院微生物研究所河南农业大学生物技术与食品科学学院中国科学院微生物研究所 北京100080SavannahRiverEcologyLabUniversityofGeorgia227GatewayDr.SC2980郑州450002北京100080

【摘要】 从渔场底泥样品中分离纯化得到5株紫色非硫光合细菌,根据分离菌株的细胞形态结构、光合内膜结构、活细胞光吸收特征以及生理生化特征,参照伯杰氏细菌分类学手册,5个分离株分别属于沼泽红假单胞菌(Rhodopseudom onas palusteris)和类球红细菌(Rhodobacter spheroides)。利用它们的纯培养物及混合培养物进行的水产养殖试验表明,光合细菌可以明显提高养殖鱼的成活率以及生长速度,罗非鱼经过2周的养殖后,加入光合细菌的试验组比没加入光合细菌的对照组(成活率80%)成活率显著提高,最高达100%;平均体长、体重分别提高了3%~5%、20%~30%,效果显著;而同时加入光合细菌纯培养物的试验组和加入混合培养物的试验组,差别不大。

【Abstract】 Five purple non-sulfur bacterial strains were isolated from the sediments of the rearing pond. According to the cultural, morphological, cellular spectra, physiological properties and inner intracytoplasmic membrane structures, strain AS-31 that was short rod and contained lamellar intracytoplasmic membrane was identified as Rhodopseudomonas palustris, and other four isolates (AS-32, DA-32, SY-32, and SY34) which were spheroid and contained vesicular intracytoplasmic membranes were identified as Rhodobacter spheroids. Pure cultures of strains AS-31 and SY-32 were used as additive in aquiculture of Tilapia sp.. After weeks of incubation, the weight and surviving percentage of experimental fishes increased about 20% than those of the control group without any additive. However, the mixed culture of strains AS-31 and SY32 have no obvious effect than that of the pure one. It demonstrated that the cultures of purple non-sulfur bacteria are of benefit to aquiculture and could be used as an additive.

【基金】 海洋“863”项目资助(No.2001AA621040)
  • 【文献出处】 微生物学通报 ,Microbiology , 编辑部邮箱 ,2005年04期
  • 【分类号】S963.73
  • 【被引频次】35
  • 【下载频次】824
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