【摘要】 从乳酸菌保健品中分离筛选到一株能作为受体菌的乳酸菌菌株COCC101,经鉴定为粪肠球菌(Enterococcusfaecali)。抗药性实验显示这株粪肠球菌对多数药物敏感或中度敏感;粪肠球菌中没有质粒存在,转化效率和电场强度有对应的正相关,最高达到2×104转化子/μgDNA,并且能广泛接受不同来源的质粒;在Nisin诱导下可表达外源的绿色荧光蛋白(GFP)。这些结果显示COCC101菌株在乳酸菌基因工程研究中有望成为受体菌。更多还原

【Abstract】 As a host strain, Lactic acid bacteria COCC101 strain, isolated from a capsule, was identified as Enterococcus faecali. The drug-resistance experiment indicated that it is sensitive or in medium sensitive to most of drugs. There is no plasmid in this strain. The transformation efficiency is in direct proportion to the voltage. The highest efficiency is 2×104 transposons/μg DNA. The strain can accept plasmids derived from different origin, and it expressed heterogeneous Green Fluorescent Protein (GFP) under the Nisin induction. All of these results indicated that the COCC101 strain is hopeful to be a host strain.更多还原