【摘要】 应用膜过滤技术对苏云金芽孢杆菌KN11增效物质回收工艺进行了改进,发现纳滤膜(200D)能完全截留KN11增效物质,通过3种膜过滤(0.1μm微滤膜、10000D超滤膜和200D纳滤膜)的总回收率达到85.5%;与常规回收比较,膜过滤回收工艺能显著提高浓缩液和粉的增效物质含量(分别为99.35U/mL,457.70U/g),同时除去了大部分的糖、氮等可溶性杂质,使浓缩液和粉保持了较好的理化性状。所配制的Bt高含量悬乳剂的效价在15645～19465IU/μL之间,含固量较低且流动性较好,Bt高含量原粉效价可达100646IU/mg。更多还原

【Abstract】 Recovering process of synergier from Bacillus thuringiensis KN-11 was improved by membrance filter technique. The results showed that the synergier from ferment liquid could be fully intercepted by the nanomembrance (200 D), and reached the total recovery rate of 85.5% (0.1 μm micromembrance, 10,000 D ultramembrance and 200 D nanomembrance). Comparing with traditional recovering technology, membrance filter recovering technology could raise content of synergier in condense liquid and powder (99.35 U/mL, 457.70 U/g ,respectively) ,get rid of most foreign substance (such as dextrose, nitrogen) and keep condense liquid and powder in good physical-chemical characteristics. The emulsion made by membrance filter recovering technology had many excellences in high activity ( 15,645-19,465 IU/μl ), low solid content and good flowing characteristics. The technical powder had high potency (100646 IU/mg).更多还原