【摘要】 利用分子梳技术对λ DNA和组蛋白的相互作用进行了研究 .通过这种简单有效的方法 ,我们将λ DNA分子拉伸到 2 6— 2 8μm ,相当于其原长 (约 16 2 μm)的 1 6— 1 7倍 .当组蛋白与DNA结合后 ,DNA分子发生凝聚现象 ,复合体的拉伸长度明显变短 ,其峰值分布在 10— 14 μm之间 .DNA 组蛋白复合体的拉伸长度与组蛋白的浓度、与碱基对和荧光染料的比例有显著的关系 .更多还原

【Abstract】 The interactions between DNA and histone are studied by using the molecular comb ing method. With this simple but effective method, λ-DNA molecules are stretch ed to a length of 26—28 μm, 1.6—1.7 times of their contour length (～16.2 μm). After DNA binding of histones, DNA molecules are condensed and the lengths of the stretched complexes become significantly shorter, with length distributi on peak lying between 10—14 μm. The lengths of the stretched DNA-histone comp lexes are closely related to the histone concentration and the mixing ratio of D NA base pair and dye molecule.更多还原