【Abstract】 The glucose oxidase (GOD) immobilized onto the surface of activated ca rbon powders at the glassy carbon electrode (GOD-C/GC) could undergo the quasi-r eversible, direct electrochemical reaction. Its formal redox potential, E0′, is almost independent on the scan rates. The average value of E0′ is (-0.467 ± 0 .002) V (vs SCE) in the pH 6.8 phosphate buffer solution. Its apparent heterogen eous electron transfer rate constant (ks) is (1.18 ± 0.59) s-1, which is much h igher than that reported previously. The dependence of E0′ on the pH of the buf fer solution indicated that the direct electrochemical reaction of the immobiliz ed GOD is a two-electron transfer reaction process coupled with two-proton trans fer. The further experimental results demonstrated that the immobilized GOD reta ined its bioelectrocatalytic activity to the oxidation of β-D(+) glucose.更多还原