【摘要】 对亚硒酸钠诱导人宫颈癌细胞(HeLa)凋亡进行了研究,分别用荧光倒置显微镜和流式细胞仪进行凋亡形态分析和DNA片断分析.研究发现Na2SeO3对HeLa细胞的凋亡作用呈现浓度依赖性.当Na2SeO3的浓度分别为21,42,63μmol/L时,流式细胞仪检测到的细胞凋亡率分别是18.9%,33.0%,58.9%.为了进一步说明凋亡过程的分子机理,分别使用Frua-2/AM和DCFH-DA两种荧光染料检测了用Na2SeO3诱导的细胞内游离Ca2+含量和活性氧(ROS)水平的变化,发现在凋亡过程中伴随有明显的胞内Ca2+和ROS水平升高,且呈现Na2SeO3浓度相关性.这表明Ca2+和ROS在Na2SeO3诱导的HeLa细胞凋亡过程中起着重要的信号传导作用.更多还原

【Abstract】 Sodium selenite-induced HeLa cells apoptosis was researched in this study.Inverse fluorescent microscope and flow cytometry were used for morphological analysis and for DNA fragmentation analysis respectively.The cytotoxic effects of sodium selenite in HeLa cells showed dose-dependency,i.e.in the presence of 21,42 and 63 μmol/L Na₂SeO₃,the apoptosis rate was 18.9%,33.0% and 58.9%,respectively.The changes of intracellular free Ca²⁺ and ROS（reactive oxygen species） content were also detected by two fluorescence probes,Frua-2/AM and DCFH-DA.The data show that both Ca²⁺ and ROS content increase in the procedure of apoptosis in a sodium selenite concentration-dependent manner,which indicates that ROS and Ca²⁺ mediate the signal transduction in sodium selenite-induced HeLa cells（apoptosis.）更多还原