Screened some fungi of producing chitin deacetylase by solid plate color reaction and liquid ferment, and studied the producing conditions of chitin deacetylase. The results showed that 26 fungi have activity of chitin deacetylase activities from the 42 fungi. At last determined Aspergillus nidulans and Absidia coerulae that have higher capacity of producing enzyme, the enzyme activity of them were 343 U·mL~(-1) and 289 U·mL~(-1) respectively. The different fungi have some optimal Carbon sources and differe...