【摘要】 为评价99Tcm标记人血清白蛋白(99Tcm-HSA)在监测脂多糖(LPS)诱导大鼠急性肺损伤(ALI)中的价值,将20只尾静脉注射99Tcm-HSA 30 min后的成年Wistar大鼠随机分为4组:正常对照组和LPS组分别由静脉注射生理盐水和8 mg/kg LPS;氯胺酮组和氨基胍组在静脉注射LPS 8 mg/kg后30 min,分别腹腔注射氯胺酮(注射剂量为4 mg/kg)和氨基胍(注射剂量为20 mg/kg)。于注射99Tcm-HSA后3 h将大鼠放血处死,测定肺通透指数(PPI)和肺湿重与干重比值(W/D)。LPS组大鼠PPI和W/D分别为95.58±11.32和5.38±0.24,均较正常对照组(6.61±0.18和4.19±0.11)显著增高(P<0.01);氯胺酮组和氨基胍组PPI分别为36.80±7.22和33.54±6.22,均显著高于正常对照组(P<0.01),但低于LPS组(P<0.01);氯胺酮组和氨基胍组间的PPI无显著性差异(P>0.05)9。9Tcm-HSA是监测LPS诱导大鼠ALI肺通透性改变的有效方法。更多还原

【Abstract】 To evaluate the value of ⁹⁹Tc^m labeled human serum albumin(⁹⁹Tc^m-HSA) in monitoring acute lung injury（ALI） induced by lipopolysaccharide（LPS） in rats,twenty adult Wistar rats are given ⁹⁹Tc^m-HSA intravenously,and are randomly divided into four groups 30 min later.The control and LPS group are given intravenous injection of 0.9% saline and LPS 8 mg/kg respectively.The ketamine and aminoguanidine group are given intraperitoneal injection of ketamine 4 mg/kg and aminoguanidine 20 mg/kg respectively just 30 min after administration of LPS 8 mg/kg.All of the four group rats are killed by blood letting at 3 h post-injection of ⁹⁹Tc^m-HSA.Pulmonary permeability index（PPI） and the ratio of lung wet weight and dry weight（W/D） is calculated.The results of PPI and W/D in control and LPS group are 95.58±11.32 and 5.38±0.24,6.61±0.18 and 4.19±0.11,respectively.The PPI and W/D in LPS group are much higher than that in the control group（P<0.01）.The results of PPI in ketamine and aminoguanidine group are 36.80±7.22 and 33.54±（6.22）,respectively.The PPI in ketamine and aminoguanidine group are lower than that in the LPS group（P<0.01）,but still higher than that in control group（P<0.01）.There is no difference in PPI between the ketamine and aminoguanidine group（P>0.05）.So ⁹⁹Tc^m-HSA is an effective tracer in monitoring ALI induced by LPS in rats.更多还原