【摘要】 本文采用PD、GPY、YES、SYE、GPM 5种不同培养基培养海藻真菌菌株PT2,并对其在不同培养基中的生长情况、代谢产物的形成及抗肿瘤活性进行分析,旨在了解不同培养基对菌株生长和活性物质合成的影响.结果显示,PT2菌株在不同培养基上的培养特征及生长速率有所不同,液体培养基发酵液抽提物的抗肿瘤活性也有较大差别,其中SYE发酵液抽提物活性最强,对KB、Raji和Hela　3种肿瘤细胞的IC50分别为 7. 6μg/cm3、2. 4μg/cm3和 3. 3μg/cm3.采用HPLC法对不同抽提物进行分析,发现它们之间的组成成分有显著差异.本文的实验结果表明,不同培养基对该菌株的生长及活性代谢产物的合成具有显著的影响.更多还原

【Abstract】 Algicolous marine fungus PT2 was cultivated on five different kinds of media which are PD, GPY, YES, SYE and GPM. The growth status, secondary metabolite production of fungus PT2 and anti-tumor activities of extracts from fungus PT2 were analyzed to study the effects of different media on growth and active metabolite production of fungus PT2. The results showed that it was different in culture characters and growth rates of PT2 on different media. There were also various inhibiting effects on tumor cells by five culture broth extracts, the activity of extract from SYE was highest in KB,Raji and Hela tumor cells, IC₅₀ was 7.6μg/mm³, （2.4μg/mm³） and （3.3μg/mm³） respectively. Culture extracts of fungus PT2 from five media were determinated by HPLC method and there was significant difference in compositions of extracts. The results indicated that there are distinct effects of different media on growth and active metabolite production of PT2.更多还原