【摘要】 对转几丁质酶和葡聚糖酶双价抗真菌基因抗病水稻七转39种植后的根际土壤微生物群落和酶活性进行了分析。研究结果表明,七转39根内生真菌和细菌数量显著低于非转基因阴性对照七丝软粘和常规水稻竹籼B,根际土壤中真菌和细菌数量也少于七丝软粘,与竹籼B数量接近。在水稻抽穗期测定,转基因水稻根际土壤过氧化氢酶、多酚氧化酶、蔗糖酶和脲酶活性以及可溶性有机质、氮、磷含量均与对照无显著差异。转基因水稻残体腐解过程中土壤腐殖酸含量变化与七丝软粘一致。与对照相比, 种植七转39未对下茬水稻的生长产生显著影响。更多还原

【Abstract】 Qizhuan 39 was a genetically modified strain of rice stemming from an indica rice cultivar,Qisiruanzhan,by accepting 2 anti-fungus genes,"RC24" (a rice chinase gene) and "β-1,3-Glu" (a alfalfa β-1,3-Glucanase gene). Preliminary analysis showed that there were fewer endophytic bacteria and endophytic fungi in the roots of Qizhuan 39. The numbers of bacteria and fungi in the rhizosphere of Qizhuan 39 were markedly lower than of Qisiruanzhan,but near to those of Zhuxian B (another non-transgenic control). In the earing period,there was no remarkable difference in the rhizospheric soil between Qizhuan 39 and the controls in concentration of soluble organic matter,nitrogen,phosphorus,and the activities of polyphenol oxidase,hydrogen peroxidase,sucrase and urease. The amount of humic acid in the soil during the rotting process of transgenic rice residue was the same as in the non-transgenic control. There was no remarkable detrimental effect on growth of rice in next season after growing Qizhuan 39.更多还原