【摘要】 本文建立了简便、快速的分离测定辣椒干中辣椒素含量的高效液相色谱法。HPLC的条件:Burospher100C18柱(I.D.250mm×4.6mm,5μm);流动相为甲醇水(70∶30,V/V);流速为0.6mL/min;检测波长为280nm。在上述条件下,辣椒素在1.01～121.2mg/L的范围内线性关系良好,线性相关系数γ=0.9994;实验结果表明,该方法的相对标准偏差在1.38%(n=6)以内,平均回收率为99.88%。更多还原

【Abstract】 A simple,rapid HPLC method was adapted to determine capsaicin in chilli (Capsicum annuum L.) with Burospher-100 C18 column(I.D.250 mm×4.6 mm,5 μm),using MeOH-H2O(70∶30,V/V) as mobile phase with an eluting rate of 0.6 mL/min and detected at 280 nm.There is a good linear relationship in the range of 1.01～121.2 mg/L for capsaicin,and the correlative coefficient is 0.9994.When the method was applied to analyse chilli extract,and the relative standard deviation is 1.38%(n=6)and the average recovery is 99.88%.更多还原