【摘要】 α- 淀粉酶抑制剂广泛存在于高等植物中,能抑制糖类在消化道内的吸收,是一种潜在的治疗糖尿病、肥胖和高血脂的药物。本文采用D10 1大孔吸附树脂,从山茱萸干果中提取得到粗提物,再经SPSepharose和SephacrylS 30 0柱层析,得到一种具有抑制α淀粉酶活性的糖蛋白(CoGP)。SephacrylS 30 0柱层析,PAGE电泳均证明该糖蛋白为单一组分;在还原条件下的SDS PAGE电泳中呈现两条蛋白带,其分子量分别是6 2 .OkD和5 6 .8kD ,利用SephacrylS 30 0柱层析测定表观分子量为15 8.9kD ,表明CoGP由两个亚基组成;利用苯酚 硫酸法测得其糖含量约为5 1% ;在α淀粉酶的抑制实验中,山茱萸糖蛋白表现出反竞争的抑制特性。更多还原

【Abstract】 A glycoprotein(CoGP)was isolated from fruits of Fructus Corni by using D 101 macro-reticular resine,ion exchange chromatography on SP Sepharose and gel chromatography on Sephacryl S-300.The glycoprotein showed to be a proteinaceous inhibitor of α-amylase.The homogeneity of this glycoprotein was proven by gel filtration on Sephacryl S-300 and PAGE.Under reducing conditions,it showed two bands on SDS-PAGE corresponding to molecular weight of 62.0 kD and 56.8 kD respectively.The molecular weight of the native glycoprotein was 158.9 kD estimated on Sephacryl S-300.The results above suggest that the glycoprotein is composed of two units.The purified CoGP’s carbohydrate content is about 51%.During preincubation of α-amylase and inhibitor,the glycoprotein inhibits porcine pancreatic α-amylase in an anticompetitive manner.更多还原