【摘要】 目的押探讨成骨生长肽羧基端片段衍生物G38I、G38K对去卵巢大鼠骨转换生化指标、骨密度和骨力学性能的影响及各指标间相互关系。方法:将3个月龄SD雌性大鼠40只随机分为5组,设假手术组(S组)和双侧卵巢切除(OVX)模型组(M组)为阴性对照,其余3组行OVX后分别予G38I、G38K和阿仑膦酸钠(ALN)治疗。术后60d处死,测血钙、磷和碱性磷酸酶,测量腰椎体和股骨骨密度(BMD),骨灰重/干重比值和骨力学指标。结果:与S组比较,M组血钙、磷升高,骨灰重/干重比值降低,经G38I、G38K治疗后上述指标恢复至S组水平,并显著升高腰椎体BMD,提高股骨弹性应力和最大应力,而股骨和腰椎体弹性模量呈升高趋势。灰重/干重比值与BMD呈正相关,BMD和力学指标间无相关性。结论:G38I或G38K的治疗可一定程度上减轻去卵巢大鼠的骨丢失,改善骨力学性能,评价抗骨质疏松药物疗效应同时关注骨量和骨质量。更多还原

【Abstract】 Objective: To study the effects of derivatives of OGP(10～14) G38I and G38K on bone metabolic characteristics, bone mineral density (BMD) and bone biomechanics in ovariectomized rats, and investigate the relationships between these indexes. Methods: Forty female Sprague-Dawley rats with three month old were randomly divided into 5 groups including sham-operated (S) group and ovariectomy model (M) group as negative control groups and other three groups with G38I, G38K and alendronate respectively after bilaterally ovariectomized (OVX). All rats were sacrificed 60 days after the operation. The serum Ca, P and ALP, the BMD of femora and lumbar vertebra, the ratio of ash weight to dry weight and the biomechanical parameters were measured. Results: Serum Ca and P increased and the ratio of ash weight to dry weight decreased in group M compared with group S. All these indexes returned back to the level of group S after treated with G38I and G38K, the lumbar vertebral BMD significantly increased, and the femoral maximal stress enhanced in group M. The ratio of ash weight to dry weight showed a positive correlation with BMD. There was no relationship between BMD and biomechanical parameters. Conclusion: The bone loss decreases and the biomechanics improves in ovariectomized rats with the treatment of G38I and G38K. It’s important to pay attention to the bone quantity and quality in the evaluation of drug effects.更多还原