【摘要】 目的:探讨骨形成肽羧基端衍生物G35I在体外对成骨细胞和破骨细胞的影响。方法:利用体外人成骨细胞和骨髓来源大鼠破骨细胞培养和RT-PCR方法进行研究。结果:(1)G35I在体外可以刺激人胚胎长骨来源的成骨细胞的增生。(2)G35I在10-7mol/L可以刺激骨髓来源的破骨细胞样细胞的诱导分化形成,而在其他浓度(10-8~10-11mol/L)此作用不明显。(3)G35I在10-7mol/L条件下可以抑制成骨细胞骨保护素的表达,且在72h作用最明显。结论:骨形成肽羧基端衍生物G35I在不同浓度时对骨代谢的两种功能细胞均有一定作用。更多还原

【Abstract】 Objective Bone remodeling was carried out by osteoclast and osteoblast. The effects of osteogenic growth peptide C-terminal derivative G35I on osteoblast and osteoclast were investigated. Methods The effects of G35I on the forming of osteoclastic like cell induced from the bone marrow cells and on the human osteoblast were investigated in vitro. Semi-quantity RT-PCR was used to examine the effect of G35I on the expression of osteoprotegerin in human osteoblast. Results 1 G35I may stimulate the proliferation of human osteoblast in vitro. 2 G35I may enhance the formation of osteoclastic like cell at the concentration of 10-7 mol/L in bone marrow cells culture system. 3 G35I may decrease the expression of osteoprogerin in the human osteoblast in culture at the concentration of 10-7 mol/L and the effect was more significant when the action went on for 72 h. Conclusion Osteogenic growth peptide C-terminal derivative G35I may have effects on both osteoblast and osteoclast at different concertration.更多还原