【摘要】 目的检测WIN55,212-2对培养的大鼠三叉神经节神经元细胞内钙离子浓度([Ca2+]i)的影响并探讨其机制。方法以Fura-2/AM为钙探针,用细胞内双波长钙荧光系统检测细胞[Ca2+]i的变化。结果WIN55,212-2(0.01～10μmol/L)可浓度依赖性地升高三叉神经节神经元细胞内[Ca2+]i,EC50为0.47μmol/L;用大麻素1型受体(CB1受体)阻断剂AM251孵育后,发现10μmol/L的AM251可明显抑制10μmol/L WIN55,212-2对三叉神经节神经元细胞内[Ca2+]i的作用(P<0.01)。结论WIN55,212-2可浓度依赖性地升高三叉神经节神经元细胞内[Ca2+]i,该作用可能是由CB1受体所介导。更多还原

【Abstract】 Objective To investigate the effects of WIN 55,212-2 on the intracellular calcium (_ i ) in cultured rat trigeminal ganglion (TG) neurons and the mechanism.Methods By using Fura-2/AM as a probe, spectrofluorometric technique was employed to determine the _i. Results WIN 55,212-2 (0.01-10 μmol/L) could increase _ i in a dose-dependent manner in rat trigeminal ganglion neurons, with an EC_ 50 of 0.47 μmol/L, and the effect could be inhibited by 10 μmol/L AM251, an antagonist of CB1 receptor (P<0.01).Conclusion WIN 55,212-2 could increase _ i in a dose-dependent manner in rat trigeminal ganglion neurons, which might be mediated by CB1 receptor.更多还原