【摘要】 目的:用间接免疫酶法检测小鼠组织内弓形虫及抗原成份,为实验研究和制备诊断试剂盒提供材料。方法:选择最佳的实验条件,包括羊抗兔IgG-HRP的浓度、反应时间的选择,观察了消化液对染色的影响,并对该方法的敏感性、特异性、重复性进行了研究。结果:羊抗兔IgG-HRP的最适浓度为1∶100。最适抗体抗原反应时间为24h,抗体与羊抗兔IgG-HRP反应时间为1h,最适显色时间为5min。间接酶法用于虫体涂片时,兔抗弓形虫血清1∶800为最高稀释度。在肝脏、脾、肺和脑均可清楚显示弓形虫感染组织内弓形虫速殖子及抗原成份,染为棕黄色。结论:本方法敏感性高、特异性强、重复性好。该方法可用于弓形虫病的病理诊断。更多还原

【Abstract】 Objective;Using indirect immunoenzymatic technique to detect TOXOPLASMA GONDII and its antigen in infected mice in order to provide material for experimental research and clinical diagnosis of toxoplasmosis.Methods;The best experimental conditions were selected,such as suitable response time,sheep anti-rabbit HRP serum dilute concentration,effect of digest liquor to stain and have studied sensitivity,specificity and repetition of the technique.Results;The suitable dilute concentration of sheep anti-rabbit HRP serum is 1∶100.The proper time of the reactions between TOXOPLASMA GONDII antigen and antibody,antibody and sheep anti-rabbit HRP serum and staining is 24 h,1 h,5 min respectively.Using to stain toxoplasma smear preparation,the highest dilute concentration of rabbit anti-toxoplasma serum is 1∶800?The dilute rabbit anti-toxoplasma serum can be kept 2 months under-20 ℃.In liver,spleen,lung and brain,the indirect immunoenzymatic technique can demonstrate tachyzoites,cyst and toxoplasma antigen clearly in the infected tissues.Conclusion;The technique has characteristics of high sensitivity、strong specificity and good repetition.The method can be used to make pathology diagnosis.更多还原