【摘要】 目的:探讨运动训练带来心血管系统ADM、RAMP2在mRNA水平上的变化。方法:选用雄性Sprague-Dawley(SD)大鼠随机分为3组,安静对照组(CR,n=8)、运动力竭组(ER,n=8)和运动训练组(TR,n=8)进行饲养训练。RT-PCR测定心尖肌组织和主动脉弓部血管ADMmRNA、RAMP2mRNA的表达水平。结果发现:(1)与安静对照组相比,一次急性有氧力竭游泳运动对大鼠心脏和主动脉ADM和RAMP2的mRNA表达无显著影响;(2)与安静对照组相比,长时期进行低强度的有氧游泳训练能在不同程度上引起心脏ADM和RAMP2的mRNA表达显著上调,反映出长期低强度有氧运动训练后心脏在分子水平上的良好适应;而长时期进行低强度有氧游泳训练对主动脉的影响是ADMmRNA表达显著下调,RAMP2、mRNA表达无显著变化。更多还原

【Abstract】 The main purpose of the research was to survey rats cardiovascular expression of ADM mRNA? RAMP2mRNA after a eight-week swimming training, and then to elucidate elementarily molecular mechanism of exercise-induced adaptation. 24 purebred male Sprague-Dawley rats were divided randomly into three groups: controlled(CR, n=8), exhausted(ER, n=8), trained(TR, n=8). After swimming training left ventricular muscle and main artery arch are extracted, we are inspecting the expression of ADM mRNA, RAMP2mRNA by RT-PCR. The results were shown as follows: 1.Comparing with the controlled group, one time exhausting exercise has no significant effect on cardiovascular expression of ADM mRNA and RAMP2mRNA. 2.Comparing with the controlled group, long-term aerobic swimming training made significantly cardiac expression of ADM mRNA and RAMP2mRNA rising, so that long-term aerobic training induces benign cardiac adaptation in molecular level; nevertheless, long-term aerobic swimming training made significantly vascular expression of ADM mRNA down, and has no significant effect on vascular expression of RAMP2mRNA.更多还原