【摘要】 基于蛋白质对聚丙烯酸修饰的纳米PbS复合粒子共振光散射的增强效应,建立了一种新的测定蛋白质的共振光散射法。在超声辐射下,用过二硫酸钾(KPS)做引发剂,用原位聚合的方法使丙烯酸原位聚合在纳米PbS上。所得的复合纳米粒子表面就覆盖了大量的羧基(—COOH),不仅增加了复合纳米粒子的水溶性和稳定性,而且使其具有良好的生物相容性。在pH=0.91的NaAc-HCl溶液中,复合纳米粒子与蛋白质结合后,使得其共振光散射强度剧增,且其增强的程度与所加入的蛋白质浓度成线性关系。在λ=385 nm处,共振光散射强度最大。在最佳条件下,建立了工作曲线,线性范围为0.2~20μg.mL-1(HSA),0.2~20μg.mL-1(Humanγ_IgG),0.15~18μg.mL-1(BSA);检出限分别为25、14、27 ng.mL-1。该法简便、快速、灵敏度高。更多还原

【Abstract】 A novel resonance light scattering(RLS)method is developed for the determination of protein.It is based on the fact that the RLS intensity of nano-PbS modified by polyacrylic acid(PAA)is significantly increased in the presence of protein.The modified nano-PbS is prepared by polymerizing the acrylic acid(AA)onto the nano-PbS in the presence of potassium persulfate(KPS)by an in situ polymerization method under ultrasonic irradiation.The surface of the PBSPAA composite nanoparticle is covered with abundant carboxylic groups(—COOH).The composite nanoparticle is water-soluble and biocompatible.It can comˉbine with protein in NaAc-HCl solution(pH=0.91)to give strong RLS atλ=385nm.Under optimum conditions,the RLS intensity is linearly proportional to the concentration of proteins.The linear range is0.2-20μg·mL-1 for HSA,0.2-20μg·mL-1 for Humanγ-IgG and0.15-18μg·mL-1 for BSA,and the LOD is25ng·mL-1 for HAS,14ng·mL-1 for Humanγ-IgG and27ng·mL-1 for BSA.The method presented here is simple,fast and sensitive.更多还原