【摘要】 采用固体铂盘电极支撑的双层类脂膜(Pt-BLM)技术固定乙肝表面抗体(HBsAb),研制成Pt-BLM-HBsAb电位型非标记免疫传感器,用于检测人血清中乙肝表面抗原(HBsAg)。对影响构筑BLM的各种因素进行了研究,用循环伏安法对Pt-BLM进行了表征。传感器对HBsAg呈Nernst响应,其线性范围为2~320μg/L,线性回归方程为ΔV=-1.09+32.4 lgρ(HBsAg),线性相关系数r=0.999 8。该传感器响应迅速,灵敏度高,稳定性及选择性好,于4℃干态保存4周,其响应信号基本不变。将其用于临床血清检验,结果满意。更多还原

【Abstract】 A new type of non-labeled potentiometric immunosensor(Pt-BLM-HBsAb)for the determination of hepatitis B surface antigen(HBsAg)was developed by immobilizing hepatitis B surface antibody(HBsAb)in the bilayer lipid membrane(BLM),which was supported by the platinum disk electrode(Pt-BLM).Effects of some experimental conditions such as the ratio of cholesterol and lecithin,species of the solvent,and the added quantum of HBsAb etc.on the potential response of the immunosensor were investigated.The immunosensor was characterized by cyclic voltammetry.The response potential of the immunosensor is linear withρ(HBsAg),over a concentration range of2-320μg/L with a correlation coefficient of0.9998and a linear regression equation ofΔV=-1.09+32.4lgρ(HBsAg).The immunosensor has a fast response time,high sensitivity,good selectivity and stability of about four weeks when storing a dry state at4℃.This method has been used to determine the real serum samples with satisfactory results.更多还原