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乌龙丹流浸膏质量标准研究
Quality Standard of Wulongdan Liquid Extract
【摘要】 目的:建立乌龙丹的质量控制方法。方法:应用TLC法对首乌、黄芪进行定性鉴别,应用高效液相色谱法测定葛根素的含量。色谱条件:流速1 m l/m in,流动相:甲醇∶乙腈∶0.025 mol/L磷酸20∶4∶76,检测波长:250 nm。结果:薄层色谱定性方法简便、灵敏、专属性强;葛根素在0.052 5~0.735μg范围之内呈良好的线性关系,r=0.999 5。平均回收率99.18%,RSD=1.55%。结论:此法简便、准确,可有效控制产品质量。
【Abstract】 Objective:To build up the quality control method for Wulongdan Liquid Extract.Methods:Qualitative determination of Radix Pueriae and Radix Polygoni Multiflori.Puerarin was carried out by HPLC.The mobile phase was ethanol-acetonitrile-0.025 mol/L phosphoric acid(20∶4∶76)at a flow of 1.0ml/min,and detection wavelength was 250nm.Results:TLC qualitative methods were simple,sensitive and specific,the determination method for puerarin had a good linear relationship in the range of(0.052 5)～0.735 μg,r=0.999 5.The average recovery was 99.18%,RSD=1.55%.Conclusion:This method is simple,accurate and can be used as the standards for quality control of this product.
- 【文献出处】 时珍国医国药 ,LiShiZhen Medicine and Materia Medica Research , 编辑部邮箱 ,2005年12期
- 【分类号】R286;
- 【被引频次】6
- 【下载频次】109