【摘要】 目的:建立妇康阴道泡腾片中没食子酸和盐酸小檗碱的含量测定方法。方法:采用高效液相色谱法,色谱柱为岛津Shampack VP ODS柱,测定没食子酸的流动相为0.2%甲醇的乙腈溶液-含0.1%三乙胺的0.1%磷酸水溶液(3∶97),检测波长为270 nm;测定盐酸小檗碱的流动相为乙腈-含0.1%三乙胺的0.05 mol.L-1磷酸二氢钾水溶液(25∶75),混合后用磷酸调pH=3,检测波长为345 nm。结果:没食子酸在0.026 04~0.208 32μg范围内呈良好的线性关系,r=0.999 6,平均回收率为97.89%,RSD=1.27%(n=6)。盐酸小檗碱在0.019 56~0.195 6μg范围内呈良好的线性关系,r=0.999 8,平均回收率为98.98%,RSD=1.39%(n=6)。结论:该法简单、快速、专属性强、重现性好,能够较好地控制产品的质量。更多还原

【Abstract】 Objective:To establish the methods for the determination of gallic acid and berberine hydrochloride in Fukang vaginal effervescent tablets.Methods:The separation was performed on Shimadzu Sham-pack VPS（150 mm×4.6 mm,5 μm） column.The mobile phase of gallic acid was acetonitrile（with 0.2% methonal）-0.1% phasphoric acid（with 0.1% triehylamine）（3∶97）.The mobile phase of berberine hydrochloride was acetonitrile-0.05mol·L^-1 potassium dihydrogen phosphate（with 0.1% triehylamine）（25∶75,mixture and adjust pH to 3.0 with phasphoric acid）.The column temperature of gallic acid was 35℃ was 25℃ of berberine hydrochloride.The detection wavelength of gallic acid was at 270 nm,and was at 345 nm of berberine hydrochloride.Results:The linear of gallinic acid was in the range of 0.026 04～0.208 32 μg,r=（0.999 6）.The average recovery was 97.89% and RSD was 1.27%（n=6）.The linear of berberine hydrochloride was in the range of 0.019 56～0.195 6 μg,r=（0.999 8）.The average recovery was 98.98% and RSD was 1.39%（n=6）.Conclusion:The mothed is convenient,rapid,reliable and accurate.It can be used for quality control of Fukang vaginal effervescent tablets.更多还原