【摘要】 目的:测定清开灵注射液、清开灵颗粒(哈尔滨、广州)中黄芩苷的含量。方法:反相高效液相色谱法。色谱柱为Kro-masil C1 8(4.6 mm×15 0 mm,5 μm) ;流动相为甲醇-水-磷酸(6 0∶4 0∶0 .2 ) ;检测波长:2 80 nm;流速:1.0 ml·min- 1 ;柱温:30℃。结果:黄芩苷对照品溶液在2 5～2 0 0μg/ ml范围内呈良好的线性关系(r=0 .9995 ) ,平均回收率为10 6 .5 3% ,RSD为1.2 % (n=5 )。结论:该法快捷、简便、准确性高,为中药复方提供了更加简便、合理、可靠的质控方法。更多还原

【Abstract】 Objective:To determine the bioactive c ompound’s content of baicalin in Qingkailing injection and Qingkailing granule ( Harbin,Guangzhou).Methods:RP-HPLC:Kromasil C 18 column(4.6 mm×150 mm, 5 μm) with methanol-water-phosphoric acid(60∶40∶0.2) as mobile phase;The fl ow rate was 1 ml/min,the colum temperature was 30℃and detection was effected at 280 nm.Results:There was a good linear relationship of the baical in and the linear range was 25～200 μg/ml.(r=0.999 5)The average recover y was 106.53%,RSD was 1.2%(n=5).Conclusion:This method is easy and rapid with good accurac y and is a more convinent, reasonable and credible quality control method for th e Chinese medicinal medicines.更多还原