【摘要】 目的:建立同时测定复方磺胺甲唑片中磺胺甲唑和甲氧苄啶含量的高效液相色谱法。方法:采用KromasilC18色谱柱(150mm×4.6mm,5μm),甲醇-水-冰醋酸-三乙胺(225∶345∶0.5∶0.2)为流动相,流速为0.7ml/min,检测波长为239nm。结果:结果磺胺甲唑在15～75μg/ml范围内,峰面积与其浓度线性关系良好(r=0.9994),平均回收率为99.4%,重复性实验RSD为0.8%(n=6),检测限为0.1μg/ml;甲氧苄啶在3～15μg/ml的范围内,峰面积与其浓度线性关系良好(r=0.9992),平均回收率为99.1%,重复性试验RSD为0.6%(n=6),检测限为0.02μg/ml。结论:该法准确、简便、快速、专属性强,灵敏度高,适用于复方磺胺甲唑片的含量测定。更多还原

【Abstract】 Objective:To establish an HPLC method for determination of SMZ and TMP in Compound Sulfamethoxazole and Trimethoprim Tablets.Methods:SMZ and TMP in the tablets were determined by HPLC with a Kromasil C 18 column(250 mm×4.6 mm,5 μm) with UV detection at 239 nm, and the mobile phase was consisted of methyl alcohol-water-acetic acid glacial-triethylamine(225∶345∶0.5∶0.2), the flow rate was 0.7 ml/min.Results:SMZ and TMP in compound sulfamethoxazole and trimethoprim tablets can be separated. The calibration curve was linear (r=0.999 4) within the range of 15～75 μg/ml for SMZ, the average recovery was 99.4% with repeatability precision RSD was 0.8% (n=6). The calibration curve was linear (r=0.999 2) within the range of 3～15 μg/ml for TMP, the average recovery was 99.1% with repeatability precision RSD was 0.6% (n=6).Conclusion:The method is simple, quick and accurate with the high sensitivity and is suitable for the quality control for SMZ and TMP in compound sulfamethoxazole and trimethoprim tablets.更多还原