【摘要】 目的观察直肠癌的淋巴管生成情况并探讨淋巴管生成在直肠癌淋巴结转移中的作用及对患者预后的影响。方法以免疫组化方法对63例有完整随访资料的直肠癌及相应切缘正常组织的淋巴管进行染色,并测定淋巴管密度(lym phatic vesse l dens ity,LVD);同时检测促淋巴管生长因子VEGF-C及其受体VEGFR-3的表达。结果直肠癌肿瘤周边区淋巴管密度(peritum ora l LVD,LVD pt)显著高于中心区淋巴管密度(in tratum ora l LVD,LVD it)及正常组织,而后两者相比差异无显著性;其中LVD pt与直肠癌VEGF-C及VEGFR-3的表达、淋巴结转移、低的5年生存率密切相关。结论直肠癌主要是肿瘤周边区存在着淋巴管的生成,淋巴管的数量不但与直肠癌的淋巴结转移密切相关而且可以作为评价患者预后的一个可靠指标。更多还原

【Abstract】 Objective To explore the role of lymphangiogenesis in lymph node metastasis of rectal cancer and the influnce to prognosis.Methods Immunohistochemistry((S-P) method) was used to mark lymphatic endothelial cells and toevaluate lymphatic vessel density(LVD).The expression of VEGF-C and its receptor VEGFR-3 in 63 cases of tumor tissues and 63 nomal mucosa were detected,respectively.Results Staining of lymphatic endothelial cells was strong and distinct.LVD in peritumoral area(LVDpt)was significantly higher than in intratumoral area(LVDit) and nomal mucosa,but there was no significant difference between the two latters.High LVDpt was related to the expressions of VEGF-C and VEGFR-3,lymph node metastasis and low 5-year survival rate.Conclusions Lymphangiogenesis appears in tumor tissues of rectal cancer but mostly in peritumoral area.Lymphangiogenesis not only plays an essential role in lymph node metastasis but also has a significant correlation with prognosis of rectal cancer.更多还原