【摘要】 目的　为临床结核性胸膜炎、腹膜炎、脑膜炎结核杆菌的检测寻找一个敏感、快速、可靠的实验检测方法。　方法　采用直接涂片、血清抗体检测和荧光定量—聚合酶链反应 (FQ -PCR )方法 ,对 85份结核患者和 13 1份非结核患者脑脊液和胸腹水标本中结核杆菌和抗结合抗体进行分析 ,其中胸水 67份 ,腹水 2 1份 ,脑脊液 12 8份。　结果　临床治疗性确诊的 85例结核性胸膜炎、腹膜炎、脑膜炎患者的胸腹水脑脊液标本 ,FQ -PCR检测出 5 3例结核杆菌检出阳性(FQ -PCR) ,阳性率为 62 .4% ,13 1例非结核患者均为阴性 ;85例结核患者胸腹水直接涂片抗酸染色镜检 11例阳性 ,阳性率仅为 13 .0 % ,13 1例非结核性标本中 ,结核抗体检出 3 6例阳性 ,假阳性率达 2 7.4%。　结论　荧光定量—聚合酶链反应 (FQ -PCR)快速诊断结核性胸膜炎、腹膜炎、脑膜炎与其他传统方法相比较 ,具有快速、灵敏、高特异性等优点 ,并能克服一般聚合酶链反应假阳性多的缺点。更多还原

【Abstract】 Objective To find a method for quick and high specific tuberculosis bacilli detection in serious membrane fluid and cerebrospinal fluid, and present a proof for clinical diagnosis. Method The tuberculosis bacillis in samples from 85 patients with tuberculosis bacilli and 131 without tuberculosis bacilli were detected with acid-fast stain and Fluorescent Quantitative-PCR (FQ-PCR) respectively. The Antibody to tuberculosis bacilli in the serum samples of these patients was detected with ELISA at the same time. Result Among 85 cases of tuberculous pleuritis, tuberculous peritonitis and tuberculous meningitis, 53 of them was proved to be positive by FQ-PCR, the positive rate was 62.4%. All of 131 non- tuberculosis bacilli cases were negative. Using acid-fast stain detection, 11 of 85 were positive, the positive rate was only 13.0%. Using ELISA to detect the antibody in samples, 36 of 131cases were positive, the false positive rate was as high as 27.4%. Conclusion Contrasted with the other traditional methods, FQ-PCR is a rapid, sensitive and high specific method in diagnosing tuberculous pleuritis, tuberculous peritonitis and tuberculous meningitis.更多还原