【摘要】 目的研究胃癌患者手术前后血清肿瘤型M2-丙酮酸激酶(tumor M2-PK)的含量变化及其与临床病理学参数的关系。方法运用ELISA法定量检测胃癌患者手术前后血清中的肿瘤型M2-PK含量的变化。结果胃癌患者血清肿瘤型M2-PK增高,明显大于对照组(P<0.05)。胃癌肿瘤切除患者手术后肿瘤型M2-PK的含量低于术前(P<0.05)。而肿瘤未切除患者手术前后的差别不明显。肿瘤≥5 cm者肿瘤型M2-PK明显高于<5 cm者(P<0.05);TNMⅢ,Ⅳ期者明显高于Ⅰ,Ⅱ期者(P<0.01);而淋巴结转移情况、肿瘤胃壁浸润深度及组织学分级对肿瘤型M2-PK水平的影响无明显差别。结论胃癌患者血清肿瘤型M2-PK水平明显升高,且初步反应肿瘤大小和病期。肿瘤的存在与否,对肿瘤型M2-PK的水平有明显影响。肿瘤型M2-PK有可能用于监测胃癌肿瘤的发生和发展。更多还原

【Abstract】 Objective To determine the serum levels of tumor type M2-pyruvate kinase(tumor M2-PK) in patients with gastric cancer before and after operation,and to elucidate the relation of their levels with clinical characteristics.Methods The serum tumor M2-PK levels were measured through the quantitative sandwich ELISA in 58 gastric cancer patients,and 16 in-patients with cholecystectomy before and after operation.Results The preoperative serum tumor M2-PK levels in patients with gastric cancer were significant higher than those in the controls(P<0.05).After the operation they decreased dramatically.But the levels in patients with unresectable tumor did not changed significantly.The bigger tumors(≥5 cm) had significant higher levels than smaller ones(<5 cm)(P<(0.05)).The levels of stage Ⅲand stageⅣ were significantly higher than that in the stage Ⅰand stageⅡ(P<0.01).No significant difference could be found in different tumor invasive depth,lymph node metastasis and histological types.Conclusion The levels of tumor M2-PK may reflect the size and stage of tumor.And it changs significantly if the tumor could be resected.It may be a promising diagnostic aid for gastric cancer.更多还原