【摘要】 目的研究双基质免疫荧光法检测抗核抗体(ANA)在风湿病中的应用价值。方法采用以喉癌上皮细胞和猴肝细胞为底物的间接免疫荧光法检测211例弥漫性结缔组织病患者(CTD)和43例其他风湿病患者的血清ANA。结果CTD中ANA的阳性率为84.1%,显著高于非CTD风湿病组的2.3%(P<0.01)。ANA阳性者可呈现不同的荧光模式。结论ANA是区别CTD与非CTD风湿病的重要指标,不同的荧光模式有不同的意义。更多还原

【Abstract】 Objective To study the significance of ANA detected by double substrates(immunofluorescence) assay.Methods Hep2 cells and monkey liver tissue were compared as substrates for detection of ANA in serum in 211 pations with CTD and 43 non-CTD subjects,using indirect immunofluoresence assay.Results The positive rate of ANA in CTD group was 84.1%,in non-CTD group was 2.3%,there was significant difference(P<0.01).There were different fluorescence mo-(dels of) ANA in the positive objects.Conclusion ANA detected by double substrates immunofluorescence assay is an important method to identify CTD and non-CTD.Different fluorescence models have different significance.更多还原