【摘要】 目的克隆人AC133-2全长基因,构建PGEZ-Term-AC133—2逆转录病毒表达载体。方法采用分段克隆的方法,通过聚合链式反应从胎肝文库中克隆AC133-2,并构建AC133—2基因的逆转录病毒表达载体。结果成功克隆AC133—2全长基因并构建PGEZ-Term-AC133-2逆转录病毒表达载体。结论AC133-2全长基因克隆及构建逆转录病毒表达载体的成功,为构建AC133-2转基因细胞和制备抗人AC133-2单克隆抗体和研究AC133-2的生物学功能奠定了物质基础。更多还原

【Abstract】 Objective To clone AC133-2, construct retroviral vector. Methods AC 133-2 was cloned from fetal liver. The gene was ligated with T-Vectoe and sequenced to construct retroviral vector constisting of AC133-2. Results AC133-2 and construction of retroviral vector was cloned successfully. Conclusion Successful cloning of AC133-2 and construction of retroviral vector lays the foundation for constructed the AC 133-2 transgenic cells, and preparation and characterization of anti-AC133-2 McAbs.更多还原