【摘要】 目的研究新生儿窒息后血清对人近曲肾小管上皮细胞(HK2)损伤的作用及其胞内信号传导机制。方法以人近曲肾小管上皮细胞(HK2)为研究对象,实验共分为对照组、窒息组和吡咯烷二硫氨基甲酸(PDTC)干预组。以20%血清浓度作为攻击浓度,采用流式细胞仪分别测定上述各组细胞的凋亡率。结果与对照组HK2细胞凋亡率[(13.3±1.70)%]相比,窒息组和PDTC干预组HK2细胞凋亡率[(46.73±3.68)%]和[(31.19±2.79)%]均增高,差异具有显著性意义(P均<0.001);与窒息组相比,PDTC干预组HK2细胞凋亡率降低,差异有显著性意义(P<0.001)。结论新生儿窒息后血清可诱导人HK2凋亡,其胞内信号机制涉及到NFκB活化。更多还原

【Abstract】 Objective To investigate the role and intracellular signal transduction mechanism in the injury of renal tubular cells induced by postasphyxial-serum in neonate.Methods Human renal proximal tubular cell(HK-2 cell) was used as target cell. The experiment was designed as:control group, asphyxia group ,and pyrrolidine dithiocarbamate (PDTC)blocking group. The attacking concentration of serum was 20%, and the apoptosis rate of HK-2 cells was detected by flow cytometer.Results Compared with controls[(13.3±1.70)%],after being stimulated with postasphyxial-serum, the apoptosis rate of HK-2 cells of asphyxia group [(46.73±3.68)%] and PDTC blocking group [(31.19±2.79)%]were significantly increased(P<0.001),but compared with asphyxia group, the apoptosis rate of PDTC blocking group was significantly decreased (P<0.001).Conclusion The postasphyxial-serum is a stimulus that may induce the apoptosis of human renal tubular cells and its intracellular signal transduction mechanism is presumably involved in the activation of nuclear factor-κB.更多还原