【摘要】 目的　探讨高压氧对全脑缺血再灌注损伤的保护作用及其机制。方法　选择 60只雄性Wistar大鼠 ,将大鼠随机分为高压氧治疗组 (H组 )和常压吸氧治疗组 (N组 ) ,采用改良四血管阻塞法制作大鼠全脑缺血再灌注损伤模型。再灌注 2 4h后 ,分别给予高压氧或常压吸氧治疗。结果　高压氧治疗组脑组织超氧化物歧化酶 (SOD)、神经元特异性烯醇化酶 (NSE)含量均明显高于对照组 (P <0 .0 5 ,P <0 .0 1)。电镜结果 :治疗组神经元、神经胶质细胞及毛细血管损伤逐渐减轻 ,而且受损伤细胞器的数量和程度均明显低于同疗程对照组。结论　高压氧治疗能明显提高缺血再灌注损伤大鼠脑组织SOD水平 ,降低自由基对大脑的损害。高压氧治疗后脑组织NSE水平上升 ,脑损伤减轻。证明高压氧治疗对缺血性脑损伤有保护作用。更多还原

【Abstract】 Objective The levels of superoxide dismutuse(SOD) and neuren specific enotase(NSE) in rats brain as well as ultrastructure of hippoca mpal tissure were searched to discuss the effect and mechanism of hyperbaric oxy gen(HBO) therapy.Methods Sixty male Wistar rats were used in this study.All rats were randomly divided into 2 groups:the group of HBO and control group.The met hod of four-vessel occlusion was used to establish the global brain eschemia rep erfusion model.After 24 hours of reperfusion,HBO and normal pressure oxygen were given respectively.Results The levels of SOD and NSE in treated groups were signif icantly higher than those in control group(P<0.05,P<0.01).Electron m icroscope showed the damage degree of neuron and glial cell and capillary in tre ated groups were decreased than that of control groups.Conclusions 1.Treatment with HBO can increase level of SOD in r at brain with ischemia reperfusion injury and HBO decrease brain damage caused b y the free radical.The level of NSE increases after the treatment with HBO and r elieves damage caused by the free radical.The level of NSE increases after the t reatment with HBO and damage degree in brain tissue decreases.The change of ultr astructure is obviously better in treated group than that in control group,The t reatment with HBO can protect the damage of brain effectively after ischemia rep erfusion. J Appl Clin Pediatr,2005,20(2):151-153更多还原