【摘要】 目的探讨多药耐药基因、多药耐药相关蛋白(MRP)、肺耐药蛋白(LRP)、谷胱甘肽-S转移酶π(GST-π)mRNA在非小细胞肺癌(NSCLC)中的共表达情况及临床意义。方法1996-10~2001-10采用逆转录-多聚酶链反应技术(RT-PCR)检测哈尔滨医科大学呼吸内科49例NSCLC组织中上述耐药基因的4种多药耐药基因共表达水平,随访生存时间,计算生存概率。结果在NSCLC中总阳性率分别为53.1%,65.3%,67.3%,83.3%。两种和(或)两种以上阳性共表达率与单一阳性表达率之间有显著差异性(P<0.05)。阳性表达率在NSCLC的不同临床分期、分化程度间无差异性(P>0.05)。随着多药耐药基因共表达种类增加,生存曲线左移,生存时间缩短。结论NSCLC的MDR现象是由多药耐药基因共表达引起的,多药耐药基因共表达种类对预后有直接影响,可作为评价预后的可靠指标。更多还原

【Abstract】 Objective To investigate the expression and clinical significance of multidrug resistance gene(MDR1),multidrug-resistance-associated protein (MRP),lung resistance protein (LRP),glutathione S-transferase-π(GST-π)mRNA in non-small cell lung cancer. Methods From Oct.1996 to Oct.2001,RT-PCR was used to investigate the mRNA expression of the above four genes.We followed up the survival time one by one and calculated the probability of survival. Results The total positive rate of the four multidrug resistance gene was 53.1%,65.3%,67.3%,83.3%.There was a significant difference between the coexpression and the single positive rate( P <0.05).The positive rate of the expression of multidrug resistance gene showed no significant difference between the different TNM stages and the different degrees of cell differentiation ( P >0.05).With the increasing expression of the MDR the curves moved to left,the survival time and probability reduced. Conclusion The coexpression of the MRG results in the MDR and affects the prognosis directly.It can be used as a criterion to evaluate the prognosis.更多还原