【摘要】 目的　探讨碱性磷酸酶(ALP)及同工酶与肾脏疾病的关系。方法　1997 -03 ~2003 -05黑龙江省医院用自动生化分析仪测定43例肾脏病患者ALP浓度,聚丙烯酰胺凝胶圆盘电泳及扫描定量测定ALP同工酶浓度。结果　按肾脏病理改变分为肾小球损伤组、肾小管损伤组和重度弥漫病变组。尿液总ALP肾小球损伤组和肾小管损伤组与对照组比较差异有极显著性(P<0 .001),组织非特异型ALP同工酶与正常对照组比较差异有极显著性(P<0. 001);尿液小肠型ALP同工酶肾小管损伤组与正常对照组和肾小球损伤组比较差异有极显著性(P<0. 001)。严重病变组尿液总ALP及同工酶与正常对照组比较差异无显著性(P>0. 05 )。结论　尿液ALP同工酶可以作为肾脏不同部位损伤的指标。更多还原

【Abstract】 Objective To investigate the correlation of alkal ine phosphatase(ALP) and isozymes with renal disease.Methods In 1997-03~2003-05 the activity of ALP was determined b y biochemical automatic analyzer in Heilongjiang Province Hospital.The activity of ALP isozymes were determined by polyacrylamide disc gel electrophoresis and s canning measurement.Results The samples from patients with renal disease were divid ed into glomerular injury group,renal tubular injury group and severe widespread damage group based on the pathological changes.The activity of ALPs in urine i n the glomerular injury group and renal tubular injury group were significantly different from those in the control group (P<0.001).The activity of tissue -unspecific ALP in urine in both groups was significantly different from those in the control group and glomerular injury group (P<0.001).The activity o f intestinal ALP in urine in the renal tubular injury group was significantly di fferent from that in the control group and glomerular injury group (P<0.001 ).The activity of ALPs and isozymes in the severe widespread tissue damage grou p was not significantly different from those in the control group (P>0.05).Conclusion Isozymes of ALP in urine can be a useful marker for different renal segments injury.更多还原