Objective: To observe osteogenesis and mineralization with trichrome staining of grinded sections in reconstruction of rat critical-sized calvarial defects using tissue engineered bone.Methods:Fourteen male adult Sprague-Dawley rats were assigned randomly into experimental and control groups. Bone marrow was obtained from left femurs and tibias of all rats under general anesthesia. In experimental group, respective autogenous osteoblasts obtained from bone marrow stromal cells were differentiated and prolif...