【摘要】 应用金标免疫层析法(GICA)研制的金标免疫试纸条对食品中黄曲霉毒素B1的检测,来确立该方法的各项技术指标。结果表明:方法的最低检测限:2.5ng/mL;金标免疫试纸条在4℃环境下可稳定10个月以上;与类似毒素AFB2、AFG2的交叉反应率分别是7.14%、6.25%;检测时间小于15min;GICA与ELISA方法的符合率达90%以上。该方法简便、快速,有较高的灵敏度,重复性好,特异性强,能定性或半定量检测食品中的黄曲霉毒素B1含量。更多还原

【Abstract】 The application of detecting AFB₁ in food in Gold Labeled Immunochromatographic Assay（GICA）was studied.Conditions for the analysis of AFB₁ in food were optimized by GICA.The results showed that the detection limit for AFB₁ was around 2.5ng/ml.The whole analysis process could be completed within 15min.The shelf life of immunostrip was also examined by performing tests at 4℃ was retained for more than 10 months.The cross-reactivity ration with similar toxin AFB₂ and AFG₂ was 7.14% and 6.25% respectively.Results showed a good correlation between ELISA kit and GICA strip.The GICA method developed in this paper would be a rapid,single step and sensitive test for qualitative detection of AFB₁ in foods.更多还原