【摘要】 目的　探讨整合素αvβ6mRNA和蛋白在卵巢上皮性肿瘤组织中的表达及与临床病理特征和预后的关系。　方法　应用半定量逆转录聚合酶链反应技术 (RT PCR)和免疫组织化学 (SP)法 ,对 5例正常卵巢组织 ,10例卵巢良性上皮性肿瘤 ,35例卵巢恶性上皮性癌 ,(其中 11例卵巢恶性上皮性癌同时取转移病灶组织 ) ,行整合素αvβ6mRNA及蛋白的定量和定位分析。　结果　整合素αvβ6基因及蛋白在正常及良性卵巢上皮肿瘤组织中不表达 ,而在恶性上皮性卵巢癌组织中出现表达 ;Ⅲ、Ⅳ期恶性上皮性卵巢癌中αvβ6mRNA表达量为 (0 735± 0 115 ) μg/ml,显著高于Ⅰ、Ⅱ期(0 5 34± 0 0 6 2 ) μg/ml;蛋白强阳性染色Ⅲ、Ⅳ期占 77 8% (2 1/ 2 7) ,显著高于Ⅰ、Ⅱ期 37 5 %(3/ 8) ,差异有显著性 (P <0 0 5 ) ;病情有进展患者 ,αvβ6mRNA表达量为 (0 72 6± 0 12 1) μg/ml,显著高于病情无进展患者 (0 5 6 5± 0 0 98) μg/ml;病情有进展患者蛋白强阳性染色占 94 7% (18/19) ,而病情无进展者仅占 37 5 % (6 / 16 ) ,差异有显著性 (P <0 0 5 )。原发病灶和转移病灶整合素αvβ6mRNA表达量分别为 (0 74 2± 0 0 19) μg/ml和 (0 75 1± 0 0 93) μg/ml;蛋白强阳性染色分别为4 5 5 % (5 / 11)和 5 4 5 % (6 / 11)更多还原

【Abstract】 ObjectiveTo investigate relationships between the expression of α vβ 6 integrin and clinical pathological characteristics and prognosis of patients with epithelial ovarian tumor (EOC).MethodsUsing reverse transcriptase ploymerase chain reaction and immunohistochemistry to detect the expression of α vβ 6 integrin in 5 normal cases,10 ovarian benign tumor cases and 35 epithelial ovarian cancer cases,while collecting 11 metastasis of the 35 EOC cases.ResultsThe de novo expression of α vβ 6 integrin could be detected in all EOC cases.Whereas α vβ 6 expression was not detectable in normal ovaries and benign epithelial ovarian tumors.In stage Ⅲ?Ⅳ of EOC,the rarion of α vβ 6mRNA/β actin (0 735±0 115) μg/ml was significantly higher than that in stage Ⅰand Ⅱ (0 534±0 062) μg/ml,and the percentage of strong staining of α vβ 6 proteins in stage Ⅲ?Ⅳ of EOC was higher(77 8 %)than that (37 5 %) in stage Ⅰand Ⅱ (P<0 05).In diseased progression,patients with the rarion of α vβ 6mRNA/β actin (0 726±0 121) μg/ml was significantly higher than that (0 565±0 098) μg/ml in remission(P<0 05),and the percentage of strong staining of α vβ 6m proteins was also higher in diseased progression (94 7 %) than that in remission (37 5 %)(P<0 05).There was no significant association between the expression of α vβ 6 and clinicpathological characteristic.No difference of α vβ 6m expression between primary and metastatasis tumors was found.ConclusionDe novo expression of α vβ 6 integrin gene and protein were detected in epithelial ovarian cancer,but not in normal ovaries and benign ovarian tumors.There was significant correlation between α vβ 6 expression and stage and progresis in EOC.更多还原