【摘要】 目的探讨p16在宫颈癌组织中的表达及其与临床病理参数和预后的相关性。方法采用免疫组织化学方法(S-P法)检测30例正常宫颈组织、20例CIN组织和52例宫颈鳞癌组织中的p16表达情况。结果在正常宫颈组织中p16蛋白基本呈阴性表达;在CIN组织及宫颈癌组织中呈过表达,过表达率分别为30.0%和63.5%。p16蛋白的表达与淋巴转移及病理分级有关(P<0.05),与年龄、临床分期、肿瘤大小、浸润深度、脉管有无癌栓无关(P>0.05);p16表达阳性患者的预后较p16表达阴性患者差,但两者生存率比较差别无显著性。结论抑癌基因p16的过表达发生在宫颈癌和癌前病变阶段,故其可作为1种肿瘤标记物用于宫颈癌的临床筛查及诊断。p16能否作为宫颈癌预后判断的指标尚待进一步研究。更多还原

【Abstract】 Objective Detecting the expression of tumor suppressor gene p16 in cervical carcinoma tissue and studying its relationship with clinicopathological futures and prognosis.Methods Expression of p16 protein was detected with SP immunochemistry technology in 30 cases normal cervix uteri tissue,20 cases cervical intraepithelial neoplasia tissue and 52 cases squamous cervical carcinoma tissue.Results The normal cervical tissue showed p16-negative expression.p16 was over expression in in cervical intraepithelial neoplasia tissue and squamous cervical carcinoma tissue.The p16 over-expression rate in CIN tissue was 30.0% and its over-expression rate in squamous cervical carcinoma tissue was 63.5%.The expression of p16 in squamous cervical carcinoma was relate to histologic grade and lymph node metastasis(P<0.05) but not relate to tumor infiltrate deepness、clinical stage age、tumor size(P>0.05);The prognosis of the postive expression of p16 protein group were not as good as the prognosis of the negative expression of p16 protein group;but statistically there was no difference between the two groups.Conclusion Overexpression of tumor suppression gene p16 was occured in cervical intraepithelial neoplasia(CIN) qand invasive cervical cancer,so its protein may be an useful biomarker for cervical carcinoma screening and diagnosis.The relationship between p16 and prognosis was not identified,it need further study.更多还原