【摘要】 目的探讨胆管癌组织中TGF-β1、TβRⅡ及p27kip1表达的相互关系及它们在胆管癌发生发展中的作用。方法应用免疫组织化学S-P法检测49例肝外胆管癌及8例正常胆总管组织中TGF-β1、TβRⅡ、p27kip1的表达,分析其与临床病理学特征的相关性。结果49例胆管癌组织TGF-β1、TβRⅡ和p27kip1阳性表达率分别为71.43%(35/49)、36.73%(18/49)和40.82%(20/49),而在正常胆管组织分别为12.50%(1/8)、100.00%(8/8)和87.50%(7/8);TGF-β1、p27kip1蛋白表达与胆管癌临床分期及转移相关,Ⅲ~Ⅳ期胆管癌的TβRⅡ阳性表达率(25.81%)明显低于Ⅰ~Ⅱ期(55.56%),P<0.05;TGF-β1及其Ⅱ型受体的共同表达与胆管癌临床分期相关(P<0.05);TβRⅡ与p27kip1在胆管癌中的表达呈正相关(γs=0.724,P<0.01);p27kip1表达与TGF-β1及TβRⅡ共同表达相关(P<0.01)。结论TGF-β1表达增高伴TβRⅡ、p27kip1表达降低提示胆管癌生物学行为不良;胆管癌中p27kip1蛋白的调控可能与TGF-β1信号转导有关。更多还原

【Abstract】 Objectives To investigate the mutual relationship between the expression of the TGF-β₁?TβRⅡ and p27^kip1 in cholangiocarcinoma and its role in the development of cholangiocarcinoma.Methods Immunohistochemical S-P method was used to examine the expression of TGF-β₁?TβRⅡ and p27^kip1 in specimens of 49 cases of cholangiocarcinomas tissue and 8 cases of normal common bile duct.The relationship between their expression and clinicopathological characters was analyzed.Results The positive rates of TGF-β₁,TβRⅡ and p27^kip1 were 71.43%（35/49）?36.73%（18/49） and 40.82（20/49） respectively in cholangiocarcinoma,and 12.50%（1/8）?100.00%（8/8） and 87.50（7/8） respectively in normal bile duct tissues.The expression of TGF-β₁ and p27^kip1 was related to the carcinoma stage and metastasis.The positive expression rate of TβRⅡ was significantly lower in stage Ⅲ and Ⅳ disease than in stage Ⅰ and Ⅱ disease（25.81% v.s.55.56%,P<0.05）.The coexpression of TGF-β₁ and TβRⅡ was negatively related with clinical stage（P<0.05）.The p27^kip1 expression was positively associated with TβR-Ⅱ expression（γ_s=0.724,P<0.01）.There was a significant relationship between the expression of p27^kip1 and the co-expression of TGF-β₁ and TβRⅡ（P<0.01）.Conclusion The overexpression of TGF-β₁ and lower-expression of TβRⅡ and p27^kip1 indicate poor biological behavior of cholangiocarcinoma.The regulation of the expression of p27^kip1 may be related with the TGF-β₁ signal transfer.更多还原