【摘要】 目的探讨等位基因杂合性丢失及微卫星不稳定性在白血病发病过程中的作用及意义。方法采用PCR-非变性聚丙烯酰胺凝胶电泳和硝酸银染色技术,选择位于7q多态微卫星标志序列引物探讨急性白血病等位基因杂合性丢失(LOH)现象。结果在D7S487位点LOH发生率为3.0%(1/33),D7S523发生率为6.1%(2/33),D7S515发生率为15.2(5/33),D7S636发生率为9.1%(3/33),在33例患者中无1例出现微卫星不稳定性(MSI)。20例正常对照标本中无1例出现LOH和MSI。结论7q区域可能存在肿瘤抑制基因(TSG)参与急性白血病的发生发展。更多还原

【Abstract】 Objective To investigate the role and significance of loss of heterozygosity(LOH) and microsatellite instability(MSI) on 7q in acute leukemia.Methods By using PCR-SSCP techniques,polymorphic microsatellite markers at 7q was used to investigate the LOH in acute leukemia.Results In patients with acute leukemia,the rate of LOH was 3.0%(1/33) on D7S487;6.1%(2/33) on D7S523;15.2(5/33) on D7S515;9.1%(3/33) on D7S636.None of 33 cases of acute leukemia(AL) showed MSI.None of 20 normal controls showed LOH and MSI.Conclusion There might be tumor suppressor genes on chromosome 7q that contribute to the pathogenesis and development of acute leukemia.更多还原