【摘要】 目的探讨血管内皮细胞生长因子(VEGF)、肿瘤微血管密度(MVD)2项指标与非小细胞肺癌各临床病理因素之间的关系以及两者之间的联系。方法采用免疫组织化学SABC法检测经病理检查确诊的68例非小细胞肺癌、13例癌旁和13例正常肺上皮组织VEGF、FVⅢ因子相关抗原的表达,对FVⅢ因子相关抗原标记的血管计数MVD。结果肺癌组织VEGF阳性表达率和MVD值明显高于正常肺上皮组织和癌旁组织;肿瘤直径≥5.0cm者MVD值明显高于直径<5.0cm者;腺癌MVD值明显高于鳞癌;有远处器官转移的肺癌MVD值明显高于无远处器官转移者;Ⅳ期肺癌MVD值明显高于Ⅰ期和Ⅱ期者,Ⅲ期MVD明显高于Ⅰ期,MVD值随TNM分期增加有逐渐增高的趋势;VEGF表达阳性的肺癌MVD值明显高于表达阴性者,并且随着VEGF表达程度的加强,MVD值增加。结论血管生成与肺癌的生长、浸润、转移及疾病进展密切相关;肺癌组织VEGF阳性表达率明显增加,是肺癌血管形成和维持的重要因子之一;MVD可作为判断非小细胞肺癌生物学行为,预测其发生发展的1项指标。更多还原

【Abstract】 Objective To assess the expression of vascular endothelial cell growth factor(VEGF) in non-small cell lung cancer(NSCLC) and investigate the correlations between VEGF,microvessel density(MVD) and clinicopathological factors of NSCLC.Methods The expression of VEGF and VⅢ factorrelated antigen were examined by SABC immunohistochemistry in 68 cases of NSCLC tissue,13 cases of tumor surrounding tissue and 13 cases of normal tissue.The number of microvessel labelled by VⅢ factor related antigen was calculated.Results The positive expression rates of VEGF and MVD in NSCLC were significantly higer than those in normal tissue and tumor surrounding tissue;MVD was significantly higher in NSCLC with a diameter of more than 5 cm than in those smaller,in adenoma than in squamous cancer,in NSCLC with distant metastasis than in those without.MVD was markedly increased in stage Ⅳ than in stage Ⅰ and Ⅱ diseases,or in stage Ⅲ than in stage Ⅰ diseases.The MVD increased in the high TNM stage.MVD was significantly higher in VEGF-positive NSCLC than in VEGF-negative NSCLC.MVD increased with the raise of the VEGF expression.Conclusion Angiogenesis is related to the growth,invasion,metastasis and development of NSCLC.The positive rate of VEGF expression is high in NSCLC and VEGF is one of the important angiogenic factors.MVD can be used to determine the biological behavior and predict the development of NSCLC.更多还原