【摘要】 目的　探讨血管内皮生长因子 C和CCR7在大肠癌中的表达及其与淋巴结转移的关系。方法　采用原位杂交和双重免疫组织化学技术,以41例大肠癌和41例癌旁正常大肠黏膜为研究对象,检测VEGF -CmRNA和CCR- 7mRNA的表达情况和微淋巴管密度(LVD )。结果　VEGF CmRNA和CCR -7mRNA在大肠癌组织中阳性表达率分别为3 4.15 % (14 / 41) ,5 8.5 4%(2 4/ 41)。VEGF CmRNA、CCR7mRNA和LVD均与大肠癌淋巴结转移密切相关(P <0 .0 5 )。结论　VEGF CmRNA、CCR7mRNA和LVD与大肠癌的淋巴结转移密切相关,联合检测其在内镜活检组织中的表达有助于预测淋巴结转移的发生。更多还原

【Abstract】 Objective To study the expression of vascular endothelial growth factor C(VEGF-C) and chemokine receptor CCR7 in colon carcinoma and to investigate the associations with lymph node metastasis of colon carcinoma and their values in predicting lymph node metastasis.Methods Hybridization in situ and immunohistochemical double staining technique were used to investigate expression of VEGF-C mRNA?CCR7mRNA and lymphatic vessel density(LVD) in 41 cases of colon carcinoma,41 cases of normal colon mucosa.Results The Positive expression of VEGF-C mRNA and CCR7mRNA were 34.15%(14/41) and 58.54%(24/41) in 41 cases of colon carcinoma.The expression of VEGF-C mRNA ?CCR7 mRNA and LVD were significantly correlated with lymph node metastasis(P<0.05).Conclusion Our study demonstrates that VEGF-C mRNA and CCR7 mRNA was involved in the colon carcinoma lymph node metastasis.The combined examination of VEGF-C and CCR7 expression in endoscopic biopsy specimens may be useful in predicting lymph node metastasis of colon carcinoma.更多还原