【摘要】 目的探讨bax、bcl-xL、bcl-xS3个基因在非小细胞肺癌组织及癌旁组织中的表达。方法以凝胶上rRNA条带的亮度为依据对后续分析用的起始RNA浓度进行调整;以组成型表达的beta-actin基因为外对照,对两类组织来源的起始RNA量进行监测;基因克隆及序列分析验证所用引物的可靠性;随后采用逆转录-聚合酶链式反应(RT-PCR)的方法对各基因在两类组织中的表达谱进行对比分析。结果在两类组织来源的起始RNA量基本一致的前提下,3个基因在成对组织中的表达谱存在明显差异,bax和bcl-xS在肺癌组织及癌旁组织中均有表达,bcl-xL基因在肺癌组织中的表达量较高。结论肺癌组织中也存在bax和bcl-xS等促凋亡基因的转录。更多还原

【Abstract】 Objective To explore the expression of three genes bax, bcl-xL, bcl-xS in lung cancer tissue and its surrounding tissue from different patients and the expression relationships among these genes. Methods The start concentration of all RNAs were adjusted for the consequent use according to the brightness of rRNA bands displayed on agarose gel. Constitutive expression of beta-actin gene was used as external control to monitor the beginning amounts of RNAs from two kinds of tissues. The reliability of all primers used for RT-PCR was confirmed by gene cloning and sequencing. The expression profiles of two genes were then analyzed in tissue parallel by applying RT-PCR. Results By using similar amount of initial RNAs, two were then analyzed in tissue parallel by applying RT-PCR. By using similar amount of initial RNAs, three genes were differently expressed both in lung cancer tissue and its surrounding tissue, bcl-xL exhibited higher expression in lung cancer tissue than in the surrounding tissue. Conclusion The proapoptosis genes bax and bcl-xS were unexpectedly transcribed in lung cancer tissues, suggesting the less effect on the cancer cure by simple apoptosis-promoting method. The transcription factor(s) that regulates this abnormal transcription would be the target site(s) for cancer treatment.更多还原