【摘要】 目的 :观察 rh TNF- α在体外对正常人精子的运动及其超微结构的影响。方法 :2 6例健康成年男性手淫法获得精液。Percoll梯度离心法得到的精子为精子模型 ,将精子密度调整为 1 0× 1 0 6 /ml;在 37℃、5 % CO2 的环境中 ,分别与终浓度为 60 pg/ml、90 pg/ml、2 70 pg/ml的 rh TNF- α孵育 ,同时设立对照组。在 0 .5 h和 4h时间段取样 ,CASA观察实验组和对照组精子的运动能力 ,TEM观察实验组和对照组精子的超微结构。结果 :与对照组相比较 60 pg/ml、90 pg/ml、2 70 pg/ml各组的精子运动能力明显降低 ( P<0 .0 1 )。精子超微结构发生程度不同的变化 :细胞膜变薄、扭曲或呈波浪状改变 ,大部分精子细胞膜不连续 ;细胞膜与顶体外膜间隙增宽 ,透亮度增强 ,顶体变厚 ,甚至可见顶体外膜破裂 ,顶体内容物流失 ;线粒体密度降低 ,透光度增强 ,严重者线粒体外形变圆 ,内有空泡形成 ;核膜与核内染色质未发生改变 ;纤维轴丝 9+ 2模式未改变。结论 :rh TNF- α可降低精子运动能力 ,同时破坏精子的顶体和膜结构而降低精子受精能力更多还原

【Abstract】 Objective: To study the effect of rhTNF-α on motility and ultrastructure of health human sperm in vitro. Methods: Twenty-six cases fresh semen samples collected by masturbation were analyzed according to WHO protocols. Semen samples from 16 healthy men were prepared using Percoll centrifugation. Sperm suspension was diluted to a concentration of 10×10⁶/ml in Ham’s, F10 medium Sperm samples were incubated with rhTNF-α solution（final concentration 60pg/ml, 90 pg/ml and 270 pg/ml,respectively） for 0.5h and 4h at 37℃ in 5%CO₂ , and comparative studies were made with a control group. 1、10μl Sperm samples were searched with CASA technique.2、Samples were scanned by TEM. Results: Significant differences were found between experimental groups（final concentration 60, 90 and 270 pg/ml） and control groups on viability, straight line velocity, curvilinear velocity, average path velocity and progressive motility of human spermin（P<0.01）. Meanwhile, ultrastructure of health human spermin experiment groups（final concentration 60, 90 and 270 pg/ml） and that in control groups have significant differences. The segmental breakages of acrosome with a show of atypical vacuoles are contents of lower electronic density. The acrosome is dilated and disrupted. The spaces widened with spillover material in between are discernible on the border. The plasma membrane is impaired. Conclusion: rhTNF-α can decrease human sperm motility function in vitro,which can destroy ultrastructure of health human sperm.更多还原